Sword ELISA Booster SUMMARY .pdf

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Sword ELISA Booster
Sword Diagnostics is developing a new plug-in performance booster for ELISA assays to enable trace
detection of biomarkers and offer researchers:
! Increased sensitivity:
10x to 30x improvement
! Increased precision at LoD:
CVs ≤5%
! Lower limit of detection:
10x to 30x improvement
! Detection of low abundance targets:
pg/mL
! Less Noise / Background
! Decreased plate to plate variability
! Generally compatible with your existing ELISA without assay re-design
The increase in sensitivity and precision enable other potential benefits:
! Detect low abundance markers
! Reduce sample volume
! Reduce assay time by decreasing incubation time
! Increase throughput moving to 384
! Greater confidence at the current level of quantitation
How it works:
The Sword ELISA Booster uses standard fluorescent microplate readers and immunoassay chemistry.
It is a proprietary alternative substrate that is converted to a Resonance Raman-active substrate in the
presence of horseradish peroxidase (HRP) and fluorescent excitation. This enhances performance of
the assay with:
1. Superior enzyme/substrate interaction at low concentrations producing more Resonance-Raman
molecules
2. High Sensitivity, Consistently Low CVs and Less Noise relative to traditional ELISA detection
chemistries
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Contact  us  to  improve  your  ELISA  sensitivity!    -­‐  cmurphy@sworddiagnostics.com  -­‐  (858)  337  7111  


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