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Our mission is to provide superior molecular detection
systems that are precise, simple and inexpensive.
GeneTAG has four patents awarded and three patents
pending for seven
novel probe systems that can identify gene mutations and
gene signatures that are indicative of infectious
diseases, drug-resistant subspecies, and mutation driven
1. Murray JL, Hu P, Shafer DA. Seven novel probe
systems for real-time PCR provide absolute single base
discrimination, higher signaling, and generic components. (2014). J Mol Diagn. 16, 627–638.
2. Storts DR. Alternative Probe-Based Detection Systems in Quantitative PCR. (2014). J
Mol Diagn. 16, 612-614.
Overview of DNA Detection Switch probe technology
GeneTAG offers 7 different DNA Detection Switch (DDS) probe systems that are specialized
for high-fidelity detection (error-checking probes) or for low-cost detection (genericcomponent probes). DDS probes employ 2 interacting components: a fluorescent labeled
probe and a nearly complementary quencher-labeled antiprobe. Probes preferentially bind to
their intended targets, liberating fluorescence. Specificity is enhanced by providing an excess
of antiprobes that intercept any incorrect hybridizations between probes and off-targets,
thereby preventing false positives. This general signaling mechanism is termed a ′DNA
Error-checking DDS probes: Conventional dual-labeled qPCR probes lack
error-checking mechanisms and cannot provide multi-temperature single base discrimination.
Our error-checking DDS probes function either as internal probes (iDDS or Flip probes) or
primer-probes (ZIPR probes), wherein each target-specific probe is combined with a slightly
mismatched, competitive antiprobe. The antiprobe blocks off-target detection over a wide
range of annealing temperatures (up to 30°C), and facilitates multiplexing. The internal
probes provide reliable single base discrimination and are also useful for validating next
generation sequencing results. ZIPR primer-probes are stringent reference probes that enable
1- or 2-color detection of targets of interest, such as gene fusions occurring in cancer. Primerprobes label all amplicons for maximum signaling. Our internal Flip probe system generates
linear amplification curves, which are useful for quantitative real-time or endpoint detection.
The signaling mechanisms of our error-checking probes are shown below:
Principles of error-checking probes:
Generic-component DDS probes:
Other DDS probe designs (Universal, Half-Universal, and MacMan probes) use
generic components that enable reduced cost and that perform comparably to
conventional dual-labeled probes. Follow the links above to learn more about
Principles of generic-component DDS probes:
GeneTAG Technology, Inc.
3781 Presidential Parkway, Suite 9
Atlanta, GA 30340