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Elabscience

®

Focus on lab research

Service for life science

ELISA
KIT

www.elabscience.com

About Elabscience
Elabscience specializes in immunodiagnostic technology for the
Life Science community. We have complete R&D and manufacture
platform and have good quality control for every kit by every lot.
We endeavor to reduce lot to lot variation to keep your experiment
results more consistent and precise. Through unremitting efforts
and development, Elabscience’s products have gained more
market share, customers are now all over the world in more than
100 countries.Our major products are Proteins, Antibodies, ELISA
Kits, CLIA Kits, Labeling Kits and related reagents.Elabscience
also provides custom services for our customers including Protein
Expression and Purification Service, Antibody Preparation Service,
ELISA Customized Service, Cell Biology Service, Molecular Biology
Service and Whole Research Project Solutions.

www.elabscience.cn

1

Focus on Lab Research, Service for Life Science.

Why choosing Elabsience?
Advantages:
High Stability
Full Range of Products
Efficient Delivery
Reliable Supply Chain
Perfect Technical Support and After-sale Service

Application areas
Hormone

Respiratory system

Cytokines & Growth
Factors

Digestive system

Metabolism

Enzyme & Kinase

Urinary system

Stem cell
Neuroscience

Apoptosis
Cancer

Innate immunity

Endocrinology
Development

Immunomodulator
Infectious Diseases
Hematology

Cardiovascular disease

Globulins

Autoimmunity

Hypersensitivity

2

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www.elabscience.com

Focus on Lab Research, Service for Life Science.

Top-selling Kits
Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0543

Human Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0010

Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0483

Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

12.5--800pg/mL

7.5pg/mL

E-EL-H0622

Human CACYBP (Calcyclin Binding Protein) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-H0130

Human COL1 (Collagen Type I) ELISA Kit

0.625--40ng/mL

0.375ng/mL

E-EL-H0851

Human CPP (Copeptin) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0043

Human CRP (C-Reactive Protein) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-H0835

Human CTXI (Cross Linked C-telopeptide of Type I Collagen)
ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H0864

Human D2D (D-Dimer)ELISA Kit

78.125--5000ng/mL

64.875ng/mL

E-EL-H0059

Human EGF (Epidermal Growth Factor) ELISA Kit

3.906--250pg/mL

2.344pg/mL

E-EL-H0064

Human ET-1 (Endothelin 1) ELISA Kit

1.25--80pg/mL

0.75pg/mL

E-EL-H2237

Human GC (Glucagon) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1908

Human GDF11 (Growth Differentiation Factor 11) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0177

Human GH (Growth Hormone) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-H0148

Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H0081

Human GM-CSF (Granulocyte Macrophage Colony Stimulating
Factor) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H5497

Human Hepc25 (Hepcidin 25) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-H1554

Human HMGB-1(High Mobility Group Protein B1)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H2532

Human IFN-α (Interferon Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0085

Human IFN-β(Interferon Beta)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0108

Human IFN-γ (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H2161

Human IgE (Immunoglobulin E) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-H0086

Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-H0103

Human IL-10 (Interleukin 10) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0105

Human IL-17 (Interleukin 17) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0253

Human IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0149

Human IL-1β (Interleukin 1 Beta) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0099

Human IL-2 (Interleukin 2) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-H2443

Human IL-35 (Interleukin 35) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0101

Human IL-4 (Interleukin 4) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0102

Human IL-6 (Interleukin 6) ELISA Kit

7.813--500pg/mL

4.688pg/mL

www.elabscience.cn
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3

Focus on Lab Research, Service for Life Science.
Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0048

Human IL-8 (Interleukin 8) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0113

Human LEP (Leptin) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-H0020

Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0134

Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H1446

Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H1451

Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H2016

Human MT (Melatonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H1386

Human NFκB (Nuclear Factor Kappa B) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H0096

Human NGAL (Neutrophil Gelatinase Associated Lipocalin)
ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H1893

Human NPY (Neuropeptide Y) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1047

Human NSE (Neuron-Specific Enolase) ELISA Kit

2.344--150ng/mL

1.406ng/mL

E-EL-H2234

Human PI (Proinsulin) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H0183

Human PIIINP (N-Terminal Procollagen III Propeptide)
ELISA Kit

23.438--1500pg/mL

14.063pg/mL

E-EL-H1476

Human PTH (Parathyroid Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H1297

Human S100B (S100 Calcium Binding Protein B) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1487

Human TG (Thyroglobulin) ELISA Kit

125--8000ng/mL

75ng/mL

E-EL-H0110

Human TGF-β1 (Transforming Growth Factor Beta 1)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0109

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0111

Human VEGF-A (Vascular Endothelial Cell Growth Factor A )
ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0048

Rat ACTH (Adrencocorticotropic Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0329

Rat ADP/Acrp30 (Adiponectin) ELISA Kit

46.875--3000pg/mL

28.125pg/mL

E-EL-R1430

Rat ANG II (Angiotensin II) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0126

Rat BNP (Brain Natriuretic Peptide) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0135

Rat CGRP (Calcitonin Gene Related Peptide) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0269

Rat CORT (Corticosterone) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-R0007

Rat EPO (Erythropoietin) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-R0167

Rat ET-1 (Endothelin 1) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-R0391

Rat FSH (Follicle Stimulating Hormone) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-R0029

Rat GH (Growth Hormone) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-R0506

Rat hs-CRP (High Sensitivity C-Reactive Protein) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0009

Rat IFN-γ (Interferon Gamma) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0016

Rat IL-10 (Interleukin 10) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

4

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-R0567

Rat IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0011

Rat IL-1α (Interleukin 1 Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0012

Rat IL-1β (Interleukin 1 Beta) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0013

Rat IL-2 (Interleukin 2) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0014

Rat IL-4 (Interleukin 4) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0015

Rat IL-6 (Interleukin 6) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-R2466

Rat INS (Insulin) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-R0582

Rat LEP (Leptin) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0026

Rat LH (Luteinizing Hormone) ELISA Kit

1.563--100mIU/mL

0.938mIU/mL

E-EL-R0025

Rat MAU (Microalbuminuria) ELISA Kit

1.563--100μg/mL

0.938μg/mL

E-EL-R0031

Rat MT (Melatonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0520

Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-R0243

Rat OC/BGP (Osteocalcin) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-R2400

Rat PCT (Procalcitonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0046

Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1)
ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-R0084

Rat TGF-β1(Transforming Growth Factor β1) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0540

Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0019

Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-R0054

Rat Tn-T (Troponin T) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M1272

Mouse ALT (Alanine Transaminase) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0160

Mouse AST (Aspartate Aminotransferase) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0068

Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0339

Mouse C5a (Complement Component 5a) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-M0053

Mouse CRP (C-Reactive Protein) ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-M0027

Mouse EPO (Erythropoietin) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-M0090

Mouse GLP-1 (Glucagon Like Peptide 1)ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0676

Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0713

Mouse IFN-α (Interferon Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0033

Mouse IFN-β(Interferon Beta)ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0048

Mouse IFN-γ (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0690

Mouse IgA (Immunoglobulin A) ELISA Kit

6.25--400ng/mL

3.75ng/mL

E-EL-M0034

Mouse IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-M0692

Mouse IgG (Immunoglobulin G) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-M0046

Mouse IL-10 (Interleukin 10) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0726

Mouse IL-12 (Interleukin 12) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0730

Mouse IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0037

Mouse IL-1β (Interleukin 1 Beta) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-M0042

Mouse IL-2 (Interleukin 2) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0043

Mouse IL-4 (Interleukin 4) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-M0044

Mouse IL-6 (Interleukin 6) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-M2614

Mouse INS(Insulin) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-M0039

Mouse LEP (Leptin) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-M0006

Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0627

Mouse MMP-9 (Matrix Metalloproteinase-9) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-M0864

Mouse OC/BGP (Osteocalcin) ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-M0051

Mouse TGF-β1 (Transforming Growth Factor β1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0641

Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0049

Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-Ch0026

Chicken IFN-γ (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch1621

Chicken IL-10 (Interleukin 10) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch0120

Chicken IL-2 (Interleukin 2) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-Ch0018

Chicken IL-4 (Interleukin 4) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch0228

Chicken IL-6(Interleukin 6) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-P0003

Porcine IFN-γ (Interferon Gamma) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-P0004

Porcine IgG (Immunoglobulin G) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-P0020

Porcine IL-6 (Interleukin 6) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-P0010

Porcine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-GP0002

Guinea pig IgG (Immunoglobulin G) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-MK2266

Monkey CIC(Circulating Immune Complex)ELISA Kit

0.313--20ug/mL

0.188ug/mL

E-EL-RB0011

Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-0028

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-0056

cAMP (Cyclic Adenosine Monophosphate) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-0083

cGMP(Cyclic GMP)ELISA Kit

0.781--50pmol/mL

0.469pmol/mL

E-EL-0030

Cortisol ELISA Kit

12.5--800ng/mL

7.5ng/mL

6

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0137

Human ACTH (Adrenocorticotropic Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0004

Human ADP/Acrp30 (Adiponectin) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-H0317

Human AMH (Anti-Mullerian Hormone) ELISA Kit

93.75--6000pg/mL

56.25pg/mL

E-EL-H0008

Human ANG2 (Angiopoietin 2) ELISA Kit

46.875--3000pg/mL

28.125pg/mL

E-EL-H0326

Human Ang-II (Angiotensin II) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0542

Human Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-0065

E2 (Estradiol) ELISA Kit

21.875--1400pg/mL

13.125pg/mL

E-EL-0045

EPI (Epinephrine/Adrenaline) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-0067

SP (Substance P) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-0072

T (Testosterone) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-0046

DA (Dopamine) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

Frequently Asked Questions
1.Some tips for ELISA failure
ELISA failure can be attributed to many factors. Many unexpected results can be avoided if the manual is
read and fully understood before starting the experiment. On identifying assay failure, you can follow tips below.
1.  Check the expiration dates of the kit, ensure that all the reagents have been stored as indicated in the
manual.
2.  Check for signs of instability or deterioration in reagent solutions, such as precipitation or discoloration and
so on.
3.  Substrate reagent should be colorless.
4.  Use clean plastic disposable pipettes, tips, and containers for reagent preparation and storage. Avoid
cross-contamination of reagents by changing pipette tips between addition of each standard, sample and reagent.
5.  Ensure that specified incubation times and temperatures have been adhered to.
6.  Ensure that no substitution of kit reagents has occurred.
7.  It is recommended that samples and standards be run in duplicate to improve accuracy.

2.Poor Precision in ELISA experiment
Possible causes

Solutions

Incomplete washing of wells

Ensure that wash apparatus is working correctly
Ensure adequate time of soaking

Inadequate aspiration of wells

Wells should appear dry after aspiration
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Unequal mixing of reagents

Ensure adequate mixing

Pipetting error

Check calculations, make new standard curve

3.Poor Standard Curve in ELISA experiment
Possible causes

Solutions

Improper dilution of standard

Use diluent in the kit as blank value.
Ensure accurate dilution of highest standard
Ensure accurate completion of 2-fold dilution series

Incomplete washing

Ensure that wash apparatus is working correctly

Inadequate aspiration of wells

Wells should appear dry after aspiration

Unequal volumes added to wells

Check pipette function;
Recalibrate the pipetter if necessary

4.High Background in ELISA experiment
Possible causes

Solutions

Insufficient washing

See washing procedures in the manual
Increase soaking time between washes

5.Too much signal in ELISA experiment
Possible causes

Solutions

Incomplete washing of wells

Ensure that wash apparatus is working correctly
Ensure adequate time of soaking

Inadequate aspiration of wells

Wells should appear dry after aspiration

Unequal mixing of reagents

Ensure adequate mixing

Pipetting error

Set duplicate in each assay

6.Standard curve achieved but poor discrimination
Possible causes

Solutions

Not enough HRP-Conjugate

Ensure correct dilution

Plate not incubated long enough

Increase Substrate Solution incubation time

Improper calculation of standard curve
dilutions

Check calculations, make new standard curve

7.Poor Duplicates in ELISA experiment
Possible causes

8

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Solutions


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