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3.2.1.1 Sample Locations...........................................................................30
3.2.2 Microcosm Preparation and Experimentation..............................................32
3.2.3 Syringe Filtration..........................................................................................33
3.2.4 DNA Extraction............................................................................................34
3.2.5 PCR Amplification........................................................................................35
3.2.6 Agarose Gel Electrophoresis.........................................................................35
3.2.7 Denaturing Gradient Gel Electrophoresis (DGGE) Analysis.......................36
3.2.8 ImageJ Software Quantification...................................................................38
3.2.9 Statistical Analysis........................................................................................39
3.3 Results..........................................................................................................................40
3.3.1 Diversity Indices...........................................................................................40
3.3.1.1 Shannon Index...............................................................................40
3.3.1.2 Simpson Index...............................................................................40
3.3.1.3 Species Evenness...........................................................................40
3.3.1.4 Species Richness............................................................................41
3.3.2 Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus Band
Intensities......................................................................................................41
3.3.3 Microcosm Experiments...............................................................................41
3.3.3.1 Experiment One.............................................................................41
3.3.3.2 Experiment Two.............................................................................45
3.3.3.3 Experiment Three...........................................................................49
3.3.3.4 Experiment Four............................................................................53
3.3.3.5 Experiment Five.............................................................................57
3.3.4 Statistical Results..........................................................................................61
3.4 Discussion and Future Research..................................................................................62
3.4.1 Discussion.....................................................................................................62
3.4.2 Future Research............................................................................................64
CHAPTER 4: CONCLUSION......................................................................................................66
REFERENCES............................................................................................................................. .68
APPENDIX A: DIVERSITY INDEX DATA ..............................................................................72
APPENDIX B: VIBRIO BAND INTENSITY DATA..................................................................75
VITA..............................................................................................................................................78

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