Step by step Guide for eC CLEM 2D 2D(2) .pdf

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Original filename: Step by step Guide for eC-CLEM_2D-2D(2).pdf
Title: PowerPoint Presentation
Author: Xavier Heiligenstein

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Step by step Guide for
eC-CLEM
2D to 2D registration

Open both light
and electron
microscopy
images
Drag and drop

Display your
images at your
convenience
On the left panel, the top part has
several tabs.
Sequence tab shows the navigator
canvas with the display zoom and
the rotation display.
Be careful, this rotation is only a
graphical display, not a
transformation calculated and
applied to your image.

Display your
images at your
convenience
On the left panel, the top part has
several tabs.

1
2

Below, the color display is tunable
(1) channel by channel (2)
Check the metadata and edit them
if necessary (3). Pay attention to the
pixel size as it defines the error
calculation estimation

3

Type « eCCLEM » in the
search bar
Click on the plugin to launch it

eC-CLEM starts
If the plugin is not already installed,
it will automatically download and
install it. No Icy restart is required
after installation

1
2

The eC-CLEM
interface is kept
simple

3

The first step is to decide whether the
final overlay will be 2D or 3D (1).
When starting from a z-stack of
fluorescence from a section, a
« beginners » preprocessing step can
be used (2). See article for more
details.
Select the source image to transform
and the target image (3)
Launch the plugin for live seed and
transform process (4)

4

Seed the point
on the target
image (1), adjust
it on the source
(2)

1

2

In 2D registration,
3 initial points are
necessary to
compute the
initial
transformation
A prior knowledge of at least 3
homology points is necessary.


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