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data interpretation .pdf

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data interpretation
Creative Biostructure now can provide a collection of tools and analysis package for biomolecular
structure determination, refinement and analysis from crystallographic, EM or NMR data. In
structural biology field, in order to reveal a dedicated and reliable structure of biomolecule,
research data is acquired, interpreted, published, reused, and sometimes eventually discarded.
Creative Biostructure helps the development of appropriate infrastructural services, ones which
make it easier and time-saving for researchers to collect, read and process the data and obtain
the expected and target structure results.
With this deliverable, the project has reached or the deliverable has contributed to the following
objectives, including but not limited to:

Crystallographic data processing and structure determination
The physical infrastructure for X-ray crystallography structural biology includes synchrotrons,
which are affordable by a nation. There are 47 in the world (lightsources.org). Each synchrotron
provides a number of beamlines for experiments, usually including several optimized for
macromolecular X-ray crystallography, often some for other structural biology techniques
including SAXS (Small-Angle X-Ray Scattering) and CD (Circular Dichroism), and usually some
beamlines for material sciences and other non-biological applications. Creative Biostructure has
access to the most modern and popularly used synchrotron X-ray resources, such as ALS, SSRL,
CAMD, Photon Factory, SSRF, etc.
EM data processing and interpretation
Improvements in microscopes, direct electron detectors, and processing software have led to a
rapid increase in the number of high resolution cryoEM structures – the “ resolution
revolution”. This has led in turn to significant investments in electron microscopes around the
world. Structural biologists in Creative Biostructure are working on harder targets: fewer single
proteins, larger macromolecular assemblies, more membrane proteins.

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