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Custom Monoclonal Antibody Services .pdf


Original filename: Custom Monoclonal Antibody Services.pdf
Title: Custom Monoclonal Antibody Services
Author: http://www.synbio-tech.com/services/custom-monoclonal-antibody-services/

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Custom Monoclonal Antibody Services

Hybridoma technology features the effective usage of innate functions of both
immune and cancer cells to generate monoclonal antibodies (mAbs). The resulting
hybridoma cell lines are produced to combat specific antigens of interest. Such
technology has forged remarkable new approaches toward therapeutic mAb
discovery, as well as disease diagnosis and prevention.
Synbio Technologies has a robust antibody drug discovery platform utilized by our
talented scientific team from both local and international antibody specialists. This
platform provides a full range of services in custom monoclonal antibody production
including immunization, hybridoma development, antibody discovery, antibody
functional screening assays, antibody engineering and antibody expression. Using
this platform, we can deliver up to 10 high-quality functional hybridoma clones to
jumpstart our customers’ antibody research.
To better serve our customers, Synbio Technologies offers our high-quality,
customizable “one stop solution” for antibody drug discovery. Our protocol is
adjustable based on the requirements of clients or the progress of the project.

Competitive Advantages
 Proprietary Immunization Technology: Compared to traditional immunogen
design, our Universal AntigenTM can induce antibodies to recognize epitopes
containing less than 5 amino acids.
 One Stop Solution: Streamlined strategy from immunization to hybridoma
generation and characterization.
 Reliable High-Throughput Screening Assays: Optimized immunization and
high-throughput screening for affinity measurements, epitope binning,
isotyping, etc.
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

 Comprehensive Functional Assay: Extensive experience in antibody affinity
ranking, affinity measurement, cross-reactivity and other in-vitro biological
analysis.

Service Specification
Milestones

Details


Immunization






Cell Fusion & Screening





Cell Screening &
Isotyping
Antibody Production
First Delivery
Functional Assay
(Optional)
Subcloning,
Amplification &
Cryopreservation




Cell fusion
Primary screening
Clones amplification / Supernatant collection
Secondary screening
Clones selection and freezing (50-100 positive
clones)
All cell isotyping based on epitope-binding groups
Bioactivity evaluation of all positive clones
(50-100 clones)



Small scale antibody production (50-100 clones,
supernatants, 1-5mg purified antibody / clone)



Supernatants / purified mAb (for in vitro functional
assay and affinity analysis)



Functional assay provided if requested



Subcloning and screening
Clone freezing (several vials for each clone)




Final delivery

Immunization of mice, transgenic mice or rat
(according to customer’s request)
Serum test
Maintain immunized (6 months)





2 subclones for each parental clone
2 cell-lines for each subclones
5mL supernatants
Full reports

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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