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CRISPR Cas9 sgRNA Vector Construction .pdf



Original filename: CRISPR Cas9 sgRNA Vector Construction.pdf
Title: CRISPR Cas9 sgRNA Vector Construction
Author: http://www.synbio-tech.com/services/crispr-cas9/crispr-cas9-sgrna-design-center/sgrna-vector-construction/

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CRISPR Cas9 sgRNA Vector Construction
The CRISPR-Cas9 system is one of the most popular types of gene editing
technology, and has been used in increasingly widespread backgrounds over the last
few years. Due to its ease of use and strong extensibility, CRISPR-Cas9 has been
applied to fields including laboratory research, biomedicinal research, and genetic
engineering of flora and fauna. This technology can also be applied to the
construction of genetically modified animal models, construction of gene-regulated
cell lines, breeding of animals and plants, repair of hereditary disease, cancer
research, and treatments of other serious diseases/screening of relevant drug
targets.
Synbio Technologies offers sgRNA plasmid construction services for several
different animal and plant species. We guarantee fast and high-quality sgRNA
plasmid construction, tailored to meet a huge variety of requests and specifications.

Advantages
All model species sgRNA design : We offer sgRNA design for 13 different species.
Many plasmid options: We can provide several validated plasmids for different
species to help you best fulfill the requirements of your experiment.
High success rate: Several target sites can be designed for different genes which
enhance the success rate of plasmid construction.
Plasmid validation service: We also provide sgRNA activity validation services, which
can enhance the success rate of experiments.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Process

Service content

Confirm species and
vector

Different species and different vectors

Target site design

Synthesis
Target cutting activity
detection
endogenous active
validation(Conditional
choice)

We usually design 3-5 target sites on common exon for
different transcription products. The important domain
should be damaged as far as possible which causes
loss-of-function in the target gene.
Common target design wesite: http://crispr.mit.edu/
Should check the target site for single nucleotide
polymorphisms (SNPs) before synthesis. (If there are
SNPs, T7E1 enzyme cannot be used to detect mutations)
SSA activity assays

in vitro cleavage activity
assay

If the target cell has a high transfection rate, like 293T
cell, genome can be extracted after 72h. Afterwards,
amplify target sequence, verify T7E1 enzyme digestion,
and validate by sequencing

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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