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DNA Sequencing & Gene Analysis .pdf


Original filename: DNA Sequencing & Gene Analysis.pdf
Title: DNA Sequencing & Gene Analysis
Author: http://www.synbio-tech.com/services/dna-sequencing-gene-analysis/

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DNA Sequencing & Gene Analysis
DNA Sequencing & Gene Analysis
Synbio Technologies provides reliable DNA sequencing services for our clients. We
offer Sanger DNA Sequencing for normal biosynthesis and cloning projects as well
as next generation sequencing (NGS) for the analysis of transcriptome and genome.
Our well-equipped and experienced technical staff guarantees you reliable gene
sequencing and analysis services. Due to our high quality standard along with
competitive prices, Synbio Technologies is the primary choice for your research
work.

Advantages of DNA Sequencing
1. Reliability: The employment of ABI 3730xl ensures a tidy FIG peak. The sequential
reading length could be 800bp at most.
2. Secrecy: You are the only one with access to your sequence. The sample will be
destroyed after the sequencing, and you will have full control over your documents.
3. Quality: Synbio Technologies has established the gold standard, Sanger
sequencing platform along with high-throughput NGS platform.
4. Experience: Technical staff have years of experience in gene sequencing, and are
able to provide helpful technical support.

DNA Sequencing & Gene Analysis Case studies: shRNA structure
sequencing
shRNA design is typically divided into two formats, the simple stem-loop shRNA
and the microRNA-adapted shRNA (Fig.1). Generally speaking, universal protocol
doesn’t work for this kind of sequence (Fig.2). However, by using Synbio
Technologies’ enhanced protocol, high quality result can be obtained (Fig.3). Due to
its hairpin structure, shRNA is difficult to sequence in gene synthesis. With ample
experience and immense talent, Synbio Tech. is well able to deal with difficult
structures such as hairpin structure, poly structure, etc..

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Fig.1 Centroid secondary structure
shRNA template data using standard sequencing protocol:

Fig.2 No correct sequencing results for the first time.
shRNA template data using Synbio Tech. protocol:

Fig.3 Perfect results after changing sequencing program.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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