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CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines .pdf


Original filename: CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines.pdf
Title: CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines
Author: http://www.synbio-tech.com/services/crispr-cas9/mammals-knockout/lentivirus-package/

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CRISPR Cas9 Lentiviral Packaging and the Construction of
Stable Cell Lines
Lentiviral vectors have a high transfection efficiency on mammalian cells and can
provide an efficient way to integrate foreign gene into host chromosome. As Cas9
protein has a longer ORF, conventional methods (electrotransfection or chemical
reagents transfection) have a lower efficiency of inserting Cas9 gene into host cell.
Hence, high-efficiency expression lentiviral vectors can be used to construct
Cas9-expressing stable cell lines.
Our lentiviral packaging service provides lentiviral expression Cas9/Cas9 Nicknase
and Cas9-expressing stable cell lines. It can also enhance the targeting efficiency of
Cas9 and enable stable gene expression in host cells, so that we can make sure the
CRISPR-Cas9 system has a higher efficiency of gene editing.

Advantages
 Higher transfection efficiency: Lentiviral vector has a transfection efficiency of
nearly 100% on hard-to-transfect cell lines (Such as neurons, primary cells, stem
cells and tumor cells, etc.).
 Large cloning capacity: The longest gene fragment that can be inserted is 8k.
 Multigene editing: It can construct Cas9-expressing stable cell lines and enable
simultaneously knock-out multiple targets in the same cell.

Cas9 lentiviral vector sequence

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Service process

Service details

Service Items

Information
requested from
clients


CRISPR-Cas9
Lentiviral
Packaging
Service



Strain that
needs to be
modified
along with
strain
information.
Information
about the
target cell line
along with cell
culture
condition.

Delivery contents





Validated cell
line.
(Monoclonal
cell line is
optional)
Complete
technical report

Turnaround
Time

Price

Inquiry

Inquiry

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines.pdf - page 1/2
CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines.pdf - page 2/2

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