PDF Archive

Easily share your PDF documents with your contacts, on the Web and Social Networks.

Share a file Manage my documents Convert Recover PDF Search Help Contact



MiniCircle DNA Preparation Service .pdf



Original filename: MiniCircle DNA Preparation Service.pdf
Title: MiniCircle DNA Preparation Service
Author: http://www.synbio-tech.com/services/molecular-biology/minicircle-dna-preparation-service/

This PDF 1.7 document has been generated by WPS Office / , and has been sent on pdf-archive.com on 06/08/2018 at 04:18, from IP address 98.126.x.x. The current document download page has been viewed 84 times.
File size: 200 KB (4 pages).
Privacy: public file




Download original PDF file









Document preview


MiniCircle DNA Preparation Service

MiniCircle DNA (mcDNA) is a group of small-circular supercoiled DNA molecules
considered to be the minimum unit of plasmid DNA required to fulfill a designed
function in an organism, such as the expression of a gene of interest. Compared with
the typical standard plasmid DNA, MiniCircle DNA lacks an unnecessary segment of
DNA commonly known as the “backbone”. The removal of the backbone part results
in a much smaller molecule which is more feasible and accessible to work with in a
laboratory setting.
Synbio Technologies specially has developed an advanced MiniCircle DNA
preparation technology to provide the high-quality MiniCircle DNA to a wide range
of scientific research institutions, academic organizations, and to global
biotechnology/biopharmaceutical companies. We accomplish this by starting with
the individual genetic sequence you request, conduct professional design and
construct high-quality and highly efficient mcDNA.
Synbio Technologies’ MiniCircle production removes the redundant backbone
sequences completely and circumvents common concerns such as antibiotic
resistance and transgenic complications. The customized mcDNA vector system will
help with multiple research requirements including transfection, DNA vaccine and
antibody production as well as pre-clinical animal studies.

Service Advantages
 GMP-Like Manufacturing Pipeline
 ISO9001/2015 Quality Management
 Efficient MiniCircle DNA Producing Procedure
 On-Time Delivery
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Service Procedure

Quality Control
Quality Control

Standard Range

Measurement Technique

Appearance

Clear and Transparent

Visual Inspection

A260/280 Analysis

1.80~2.00

UV Spectrophotometer

Concentration

Up to 5mg/ml, default
1±0.05 mg/ml

UV Spectrophotometer

MiniCircle Percentage

>95%

Density scan on agarose gel

Supercoil Percentage
Analysis

>95%

Density scan on agarose gel

Plasmid Precursor Residue

Not Detectable

Visual inspection on
agarose gel

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

RNA Residue Analysis

Not Detectable

Visual inspection on
agarose gel

Genomic DNA Analysis

Not Detectable

Visual inspection on
agarose gel

Sterility Assurance

No clone growth on LB
plate > 48h

Incubate products on LB
plate for >48h

Restriction Enzyme
Analysis (optional)

As expected with no
other minor band

Incubate with enzyme and
analyze on agarose gel

Sequence Verification
(optional)

Correct

DNA Sequencing

Endotoxin Analysis
(optional)

<0.005EU/μg or lower

Endotoxin Analysis Assay

Featured Services

Starting Materials (One of the
following

1.Your request gene sequence (extra synthesis
fee)
2.10ng DNA sample (diluted with ddH2O or
TE buffer)
3.Colonies (fresh) or bacteria stored in
glycerol

Quantity

0.5mg,1mg,2mg,5mg

Edotoxin

<0.005EU/µg

Price

Quote

Turnaround Time

4-6 weeks>

Quality Control Methods

Basic quality control tests>

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Prepared MiniCircle DNA
Certificate of Analysis (COA)
QC Report

Standard Delivery Package Contains

Note: The price system is based on high copy number plasmids (yield > 0.1mg
plasmid per 1 g cell paste). If the pilot yield estimation assay produces < 0.1mg
plasmid per 1 g cell paste, the price is subjected to change after discussion with the
customer.

Related Services
 Syno®2.0 Gene Synthesis
 Syno®3.0 Gene Synthesis
 Synthetic DNA library
 PCR Cloning& Subcloning
 Site-Directed Mutagenesis
 Protein Expression and Purification

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


MiniCircle DNA Preparation Service.pdf - page 1/4
MiniCircle DNA Preparation Service.pdf - page 2/4
MiniCircle DNA Preparation Service.pdf - page 3/4
MiniCircle DNA Preparation Service.pdf - page 4/4

Related documents


PDF Document minicircle dna preparation service
PDF Document crispr cas9 sgrna vector construction
PDF Document crispr cas9 system activity detection
PDF Document ready to use crispr cas9 sgrna synthesis
PDF Document synthetic dna libraries case studies
PDF Document yeast protein expression


Related keywords