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Plasmid DNA Preparation.pdf


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2. Restriction enzymes were used to verify the sequence of the mini-prepped
plasmid.
3. Large volume bacteria culture or fermentation was performed to obtain a
sufficient amount of cell paste.
4. The cell paste was lysed to release plasmid DNA. The crude lysis solution was then
purified through several steps to dispose of impurities.
5. The bulk DNA solution was quality tested which included: restriction enzyme
digestion, sterility tests and endotoxin level tests.
6. After passing all of the quality control tests, the bulk DNA was formulated,
dispensed, labelled and finally delivered.
Quality Test Results:
1. Endotoxin Test: The negative control of endotoxin testing will not concrete, while
the positive control will concrete. These samples were not concrete (Fig.1), proving
that the endotoxin was successfully removed.

Fig. 1 Endotoxin testing, from left to right: negative control, positive control, sample,
endotoxin standard 5UR/mg
2. Sterility Test No bacterial colony was detected after 48 h culturing in
non-resistant medium (Fig.2).

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