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Bacterial Expression System .pdf


Original filename: Bacterial Expression System.pdf
Title: Bacterial Expression System
Author: http://www.synbio-tech.com/services/protein-engineering-platform/prokaryotic-protein-expression/

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Bacterial Expression System
The E.coli as the host cell is typically used for the prokaryotic expression system for
several reasons. The cells have advantages such as rapid proliferation, high
expression, easy purification, good stability, strong anti-pollution ability and cost
effectiveness. This is why the bacterial expression system is not only the most
mature and reliable, but also the most commonly used, economical and effective
option for protein expression.
However, there are some difficulties with the prokaryotic expression system. For
example, current codon usage bias in CDS or carrier regulatory elements are
incompatible with the host bacteria. This may lead to the failure of interest protein
expression or low expression. Another issue that may arise is that even if the
expression of interest protein succeeds, it may not be soluble.
With years of experience in protein expression and purification, Synbio Technologies
has developed a cost-effective bacterial expression platform to deliver high purity
proteins to our customers. By using the NGTM Codon Optimization Technology, the
expression level in bacterial can significantly improve. We also offer the Syno®
Guaranteed package, which guarantees 3-5 mg recombinant protein of >85% purity
to be delivered to our customers.

Competitive Advantages
 NGTM Codon Optimization Technology : Increase the protein expression level
in most host cells
 Syno® Express Vector Series : Optimized expression vectors designed to
improve the expression level
 Guaranteed Package : Deliver 3-5 mg protein of 85% purity, starting at $1,980
 One-Stop Services : Streamlined process from gene synthesis and codon
optimization to protein expression and purification

Service Procedure

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Service Specifications
Service

Description

Deliverables

Timeline

Price

3-5 µg of
Starting at 7 Starting
Gene Synthesis Codon Optimization
lyophilized plasmid Business
at
(optional)
Gene Synthesis
DNA
Days
$0.25/bp


Protein
Expression &
Purification

Syno® Guaranteed
Package





Standard Package


Endotoxin
Removal

Endotoxin level can
reach 0.1EU/μg,
upon request

Large-scale
Protein
Production

From milligram to
gram level






3-5 mg
protein
>85% purity
COA
Purified
Protein
COA
Endotoxin
Testing
Report
Purified
Protein
COA

6-8 weeks

$1,980

Starting at 6
Weeks

Quote

1-2 weeks

Quote

Quote

Quote

For those proteins with further special requirements, Synbio Technologies provides
you a variety of post processing options. These options include protein tag removal
after purification, filtration sterilization, endotoxin removal, freeze-dry, N-terminal
sequencing and other services.
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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