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Baculovirus Insect Cell Expression System .pdf


Original filename: Baculovirus-Insect Cell Expression System.pdf
Title: Baculovirus-Insect Cell Expression System
Author: http://www.synbio-tech.com/services/protein-engineering-platform/baculovirus-protein-expression/

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Baculovirus-Insect Cell Expression System
The Baculovirus-Insect Cell Expression System is one of the most popular eukaryotic
expression systems for research and industry applications. There are several
advantages of using the baculovirus-insect cell expression system, such as improved
solubility, the ability to incorporate large DNA fragments, and comprehensive
post-translational modifications.
With our experienced scientific team, Synbio Technologies has developed our own
baculovirus-insect cell expression technology platform. The production scale of this
platform is up to 500 L. Combined with our advanced protein purification facilities,
we can deliver recombinant protein products with purity 95% and above. Protein
expression levels and success rates can be significantly improved using our
proprietary NGTMCodon Optimization software.

Baculovirus-Insect Cell Expression System Advantages
Proprietary NGTMCodon Optimization Technology: significantly improve protein
expression levels and success rate.
Multiple purification strategies: affinity chromatography, ion exchange,
hydrophobic chromatography, gel filtration, etc., providing diversified choice of
protein purities.
Comprehensive protein
MALDI-TOF, HPLC, etc.

identification

services:

SDS-PAGE, Western

Blot,

Service Specifications
Steps

Specifications

Deliverables

Timeline

Gene synthesis
(optional)

NGTMCodon Optimization
Gene synthesis

Plasmid DNA

1-2 weeks*

Subcloning

Subcloning the target gene
into expression vector

Constructs

1 week

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Baculovirus
preparation

Bacmid DNA preparation and
infecting insect cell

5ml,>107pfu/ml
baculovirus

3-4 weeks

Pilot protein
expression and
purification

Pilot protein expression and
purification
Test the expression level

Expression report

1-2 weeks

Large scale protein
expression and
purification

Scale-up expression and
purification

Recombinant
protein
QC report

1-2 weeks

* The delivery time of this step bay vary due to the length and/or difficulties of the sequence(s).
Please contact our project manager for a quote.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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