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Mammalian Cell Expression System .pdf


Original filename: Mammalian Cell Expression System.pdf
Title: Mammalian Cell Expression System
Author: http://www.synbio-tech.com/services/protein-engineering-platform/mammalian-protein-expression/

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Mammalian Cell Expression System
The Mammalian Cell Expression System is an ideal system for functional protein
expression for several reasons. One of the most important reasons is biological
activity of recombinant proteins produced by this system closely resembles that of
natural proteins. For this reason, mammalian cell expression systems are widely
used in the biopharmaceutical industry.
With our experienced scientific team, Synbio Technologies has developed our own
mammalian cell expression technology platform, capable of delivering recombinant
proteins from the milligram level to the gram level. Combined with our advanced
protein purification facilities, we can deliver recombinant protein products with
purity 95% and above.

Competitive Advantages
 Proprietary NGTM Codon Optimization Technology: Significantly improve
protein expression levels and success rate.
 Recombinant Antibody Production: IgG/IgM/IgE/IgD, scFv, Fab, VHH,
guarantees 1-3 mg, >85% purity.
 Multiple Purification Strategies: Affinity chromatography, ion exchange,
hydrophobic chromatography, gel filtration, etc; Provide diversified choice of
protein purities.
 Comprehensive Protein Identification Services: SDS-PAGE, Western Blot,
MALDI-TOF, HPLC, etc.

Service Specifications
Steps

Specifications

Deliverables

Timeline

Gene synthesis
(optional)

NGTMCodon Optimization
Gene synthesis

Plasmid DNA

1-2 weeks*

Subcloning

Subcloning the target gene
into mammalian cell
expression vector

Constructs

1-2 Weeks

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Pilot protein
expression and
purification

Pilot protein expression and
purification
Test the expression level

Expression report

2-3 Weeks

Protein Expression
and Purification

Scale-up expression and
purification

Purified protein
QC report

2-3 weeks

* The delivery time of this step may vary due to the length and/or difficulties of the
sequence(s). Please contact our project manager for a quote.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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