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Pathway Synthesis .pdf



Original filename: Pathway Synthesis.pdf
Title: Pathway Synthesis
Author: http://www.synbio-tech.com/services/pathway-synthesis/

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Pathway Synthesis

Metabolic Pathway Synthesis and Library Assembly Services
Since the birth of synthetic biology in 2000, metabolic engineering and synthetic
biology research have made increasingly huge progress on high value-added natural
products. The synthesis and construction of metabolic pathways as well as diverse
DNA assembly technologies play a fundamental role in microbiology, biochemistry,
and many other relevant fields. With the rapid development of gene synthesis and
assembly technology, especially chip-based DNA synthesis technology, the price of
large DNA fragment or plasmid synthesis is decreasing year by year, providing
opportunities and access for synthetic biologists and metabolic engineers to more
easily conduct their research. Over the last 10 years, the continuous development of
high flux and automated high-throughput screening methods significantly help to
improve the convenience and simplicity of the late screening of library technology,
provide more beneficial tools for metabolic engineering and synthetic biology
research, and play an important part in chemical synthesis, pharmacology,
agricultural, and environmental fields.
 In 2003, Jay Keasling’s team reconstructed the biological synthesis pathway of
arteannuinic acid in E. coli, providing functional confirmation for this metabolic
process. Over the next decade, they would actively conduct host selection and
optimization, taking efforts to achieve product industrialization.
 In 2010, Gregory Stephanopoulos’s team reconstructed the synthesis pathway
of Paclitaxel, its precursor, and 5α-hydroxyl paclitaxel, and verified the key four
steps during this process, successfully optimizing the metabolic engineering
pathway of the Paclitaxel precursor.
 In 2015, Christina Smolke’s team successfully synthesized the precursors of
clinically opioid analgesic drugs such as thebaine and hydrocodone in yeast.
Based on Syno®2.0 and Syno®3.0 gene synthesis tools and strong long DNA
assembly capability, Synbio Technologies is confident in providing professional
technical support for metabolic pathway synthesis and library assembly. Specific
services include metabolic pathway synthesis and customized metabolic pathway
library assembly.

Featured Services
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

 Our Syno®2.0 platform is able to provide a fast synthesis of gene fragments and
modular genes;
 Our Syno®3.0 high throughput synthesis platform can assure large-scale
reserve of promoters, nucleic acid binding sites, terminators and so on.
 Reliable synthesis of long DNA; a single metabolic pathway has been
synthesized to 20Kb
 Wide range of library assembly technologies, allowing for customized library
storage

Synbio Technologies case
In this case, the diversity of the promoter library determines the final diversity of the
constructed library. The theoretical library capacity is 100, with 100 times coverage,
leading to the production of 10,000 clones. Furthermore, the PCR test of the
bacterial solution showed 78% positive and 100% accuracy.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Turnaround Time and Price
Services

Details

Turnaround
Time

Customized
Metabolic
Metabolic
<10Kb, 4-6weeks
Pathway
Pathway Synthesis >10Kb, Inquiry
Synthesis
Services
Customized
Metabolic
Metabolic
Pathway
Pathway Library
Library
Assembly
Assembly
Services

Delivered Product
Plasmids with
synthesized metabolic
pathway and glycerol
bacteria

Inquiry

Plasmid library after
Varies depending assembly and glycerol
on the pathway bacterial library,or
requested
monoclonal plasmids
and glycerol bacteria.

Inquiry

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Price

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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