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Yeast Protein Expression .pdf



Original filename: Yeast Protein Expression.pdf
Title: Yeast Protein Expression
Author: http://www.synbio-tech.com/services/protein-engineering-platform/yeast-protein-expression/

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Yeast Protein Expression
Yeast protein expression
Prokaryotic expression is mature and easy to be operated, but when it comes to the
proteins of eukaryotic organism, error folding is occurred frequently since
glycosylation and folding partner are involved. Fortunately,the expression system of
pichiapastorisis complementary to this situation to a certain extent. Relying on
powerful synthetic biology technology platform and industry-leading codon
optimization software NGTM Codon, Synbio Tech. provides you high-quality and
considerate yeast expression protein services with more than 10 years’ experience in
protein expression and purification, which can effectively save your cost and time.

Services
1.Expression vector construction (optional)
First, according to your specific requirements, we will analyze the gene sequence of
protein of interest. Through the industry-leading NGTM Codon optimization
software, the amount of gene expression can be increased by several to tens of
times. Then, gene synthesis was conducted and the recombinant plasmids are
verified by sequencing to see whether the sequences are 100% correct. Synbio Tech.
provides the service of directly cloning genes into commercial expression vectors
such as methanol-induced pPICZα、pPIC9K or component pGAPZα, etc.. In addition,
you can also choose the peculiar yeast special expression vector of Synbio Tech.to
carry on the expression test.
Delivery contents: 1 tube of freeze dried plasmid (4ug/tube) and 1 tube of glycerol
containing the plasmid TOP10
Delivery TAT: 10-20 business days
2.Linearized vector andelectricity transforming into pichiapastoris
You can also give us a constructed recombinant plasmid (please confirm the codon
of the sequence is optimized). For those verified recombinant plasmids, we choose
suitable enzymes to carry out the linearization and adopt the electric transfer
methodto transform them into the pichiapastoris. After the screening, we select at
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

least 6 colonies for expression laboratory scale test.
Delivery contents: screening report of positive colonies
Delivery TAT: 5 business day
3.Optimization of expression conditions
During the process oflaboratory scale test , we will optimize and testthe culture
conditions which are suitable for various strains, including temperature, culture
medium, inducer type, induction time, induction quantity and other factors.It is
tacitly approved thatwe use SDS-PAGE or Western blotto (optional, the customer
provides a varified first antibody) to analyze the expression effect.
Delivery contents: A copy of laboratory scale test verification report
Delivery TAT: 5 business days
4.Amplified cultivation,expression and purification interest protein
To optimize and obtain the strains with appropriate expression conditions and use
1L or greater culture scale to induce recombinant yeast expression. After the
induction, one step or multi-step purification is conducted.we use SDS-PAGE to
verify the purification andWestern blotto (optional, the customer provides a varified
first antibody) to verify the accuracy.
Delivery contents: 1-10 mg recombinant proteins and purification report data
Delivery TAT: 10-15 working days
For proteinswith tags, youcan choose remove service (optional).
5.Protein post processing (optional)
For those proteins with further special requirements, Synbio Tech. provides you a
variety of post processing options, including protein tag removal after purification,
filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing and
other services.Our experienced engineers can provide tailor-made post servicesfor
your special needs.
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Delivery contents: inquiry
Delivery TAT: inquiry

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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