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Large Scale Oligo Synthesis .pdf



Original filename: Large-Scale Oligo Synthesis.pdf
Title: Large-Scale Oligo Synthesis
Author: http://www.synbio-tech.com/services/large-scale-oligo-synthesis/

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Large-Scale Oligo Synthesis

DNA oligos are chemically synthesized with four deoxyribonucleotides of A, T, C and
G. These sequences and are widely used in various ways in field of biology. Synbio
Technologies is confident in our ability to provide high-quality DNA oligo products
to scientific researchers and industrial customers around the world. With the
combination of our state-of-the-art synthesizer. advanced production technology
and professional technical support team we have consistently achieved low
mutation rates, short turnaround times, high cost performance.
Synbio Technologies provides unmodified DNA oligo synthesis for large-scale
orders only (at least 96 DNA Oligos). These oligos may come in the form of standard
oligos, long oligos, degenerate primers, etc. Our regular synthesis scale is 2 OD for
DNA oligos. But we can also offer a flexible synthesis scale to meet customers’ PCR
or sequencing application needs. The tailored specification of the single type oligo
can reach 1-200 nmol (about 0.5-100 OD). Synbio Technologies deliveries the
large-scale oligo synthesis in 96-well and 384-well plate formats upon request.
Competitive Advantages


High-Quality: in accordance with the stringent quality control standards for
oligo synthesis, we can achieve extremely low mutation and low error rates



Highly-Customizable: Flexible synthesis scales are available, four alternative
purification options include desalt, HPLC, PAGE plus and PAGE



Technical Support: Professional teams experienced in oligo synthesis and
various modifications offer support throughout the production progress



Cost Effective: Competitive prices and affordable services

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Service Specifications
Standard Oligos
Synbio Technologies provides a fast and convenient large-scale standard
unmodified DNA oligo synthesis. Bulk custom DNA oligos can be ordered with the
same high purity, competitive prices, and fast turnaround.
Price per Base for Oligos

Length

11-60 bp

Extra Fee

Purification 50 nmol 100 nmol 200 nmol 1 µmol 5 µmol 10 µmol

Desalt

$0.31

$0.36

$0.63

$1.40

$6.55

$11.99

PAGE

$28.00

$32.00

$48.00

$84.00 $420.00 $732.00

HPLC

$30.40

$30.40

$46.40

$75.20 $172.00 $252.00

Doual
HPLC

$45.60

$45.60

$69.60

$112.80 $258.00 $378.00

* Production time applies for non-complex sequences. Please inquire if you prefer
other purification method.
Degenerate Primers
Genetic codes have the tendency to degenerate, which leads to the use of
degenerate primers in PCR experiments to exhibit specific genes. Synbio
Technologies provides both the design and synthesis of these degenerate primers,
as well as custom degenerate oligo pool synthesis upon request.



Standard Service: No design fee, suitable for more than 95% PCR projects.
Superior Service: Utilized for certain projects sensitive to codon bias. Synbio
Technologies’ professional technology can significantly reduce the deviation

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

inherent in the synthesis procedures and realize the accurate proportional
control.

Symbol Base Type

Standard Ratio

R

A,G

50% : 50%

Y

C,T

50% : 50%

M

A,C

50% : 50%

K

G,T

50% : 50%

S

C,G

50% : 50%

W

A,T

50% : 50%

H

A,C,T

33% : 33% : 33%

B

C,G,T

33% : 33% : 33%

V

A,C,G

33% : 33% : 33%

D

A,G,T

33% : 33% : 33%

N

A,C,G,T

25% : 25% : 25% : 25%

Price
Standard Level Superior Level

Free

Inquire

* Non-standard base proportion should be declared clearly, for example,
N(20%A:30%C:40%G:10%T).
Long Oligos
Based on the solid-phase oligo chemically synthesis technology, as the length of the
synthetic sequence increasing, the undesired bands of N-1 type impurities are more
unavoidable in the electrophoresis. However, some experiments still need to use
long chain genes or ultra-long oligos (130-150 mer). In doing so, the synthesis
efficiency and purification are pretty crucial.
Synbio Technologies’ Syno®1.0 Oligo Synthesis Platform utilizes an optimized
ultra-long chain design and synthesis program to meticulously monitor and control
the synthesis process. This process allows us to generate long high-quality oligos
(90-129 mer) and even ultra-long oligos (130-150 mer) according to individual
requests.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Length

Quantity

90-109 mer

1 OD

110-129 mer

1 OD

130-150 mer

0.5 OD

Purification

Turnaround Time

Price

5-6 business days
PAGE / Dual-PAGE

5-7 business days

Inquire

5-8 business days

Standard deliverables
 Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and
384-well plate
 Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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