PDF Archive

Easily share your PDF documents with your contacts, on the Web and Social Networks.

Send a file File manager PDF Toolbox Search Help Contact



Synthetic DNA Libraries Case Studies .pdf


Original filename: Synthetic DNA Libraries Case Studies.pdf
Title: Synthetic DNA Libraries Case Studies
Author: http://www.synbio-tech.com/services/synthetic-dna-libraries/synthetic-dna-libraries-case-studies/

This PDF 1.7 document has been generated by WPS Office / , and has been sent on pdf-archive.com on 13/08/2018 at 10:01, from IP address 98.126.x.x. The current document download page has been viewed 64 times.
File size: 150 KB (2 pages).
Privacy: public file




Download original PDF file









Document preview


Synthetic DNA Libraries Case Studies
Synbio Technologies’ synthetic DNA library services offer an efficient and effective
method for protein engineering and directed evolution research. We are capable of
designing and constructing a wide range of synthetic DNA libraries such as alanine
scan library, site saturation library, random substitution library, modular substitution
library to satisfy the requirements of experimenters. Synbio Technologies has
extensive experience with designing and generating these libraries which has
allowed us to supply our customers with high-quality synthetic DNA libraries all over
the world.

1. Site Saturation Library

Synbio Technologies has successfully constructed a custom site saturation library
based on our comprehensive design and synthesis technologies. For example, three
consecutive bases were mutated into the NNK formatted type at any regions of the
sequence. A site saturation library with the diversity of 108 was successfully
delivered in lyophilized plasmid form in an effective timeframe.

2. Random Substitution Library

Synbio Technologies’ random substitution libraries successfully mutated the specific
parts as showing and generated 1-4 mutations/1,000bp. According to the calculation,
the mutation rate of each clone was 1.5-2.5/kb. The schematic diagram displayed
above shows the mutagenesis frequency of different numbers of mutated bases.

3. Controlled Library
Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Synbio Technologies designed and conducted controllable mutagenesis at different
CDR regions of a targeting antibody. A controlled library was successfully produced
with our proprietary PermutationTM Technology. Using this technology, we
successfully mutated the triplet codons to change the amino acids in the target
protein sequence. Synbio Technologies’ customized controlled library can accurately
control the types and the proportion of amino acids.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


Synthetic DNA Libraries Case Studies.pdf - page 1/2
Synthetic DNA Libraries Case Studies.pdf - page 2/2

Related documents


PDF Document synthetic dna libraries case studies
PDF Document synthetic dna libraries
PDF Document site directed mutagenesis services
PDF Document chip based dna synthesis
PDF Document pathway synthesis
PDF Document antibody affinity maturation service


Related keywords