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Synthetic DNA Libraries .pdf


Original filename: Synthetic DNA Libraries.pdf
Title: Synthetic DNA Libraries
Author: http://www.synbio-tech.com/services/synthetic-dna-libraries/

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Synthetic DNA Libraries
Synbio Technologies is equipped with the world leading Syno® DNA technology
platforms ranged from standard gene synthesis to the proprietary next generation
Syno® 3.0 synthesis technology. We are capable of generating high quality DNA
libraries of any size and complexity with an efficient timeframe and lower cost. Our
synthetic DNA libraries can facilitate your various research interests: spanning from
functional genetics to directed evolution, especially to protein engineering and
antibody studies. In addition, Synbio Technologies’ experienced bioinformatics team
can help to analyze and provide synthetic DNA libraries design to your researches.

Competitive Advantages
 Generation of Multiple Library Types: Scanning, degenerate codon, triplet
codon, site saturation, random substitution, modular etc.
 Professional Technical Platforms: The flexible Syno® 2.0 and 3.0 synthesis
platforms guarantee
 a rapid and high throughput gene synthesis providing a powerful ability to
successfully assemble large DNA fragments with 100% sequence accuracy.
 Flexible Deliverables: Oligo pools, PCR fragments, pooled clones or error-free
separated colonies.
 Achievable Library Diversity: We can offer the library diversity of up to 1014
variants.

Applications of Synthetic DNA Libraries


Alanine Scan Library : Research on amino acid residues critical to protein
function, interaction and shape by replacing an individual amino acid, with
alanine, at every position.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com



Site Saturation Library : Substitute all 20 possible amino acids at any site to
evaluate structure-function relationships and to generate improved protein
variants.



Random Substitution Library : Substitute bases with triplet, random or
degenerate ones to identify locations for specific analysis.



Antibody Library : Create a high diversity synthetic antibody library or
improve existing antibody functionality such as specificity, immunogenicity,
affinity, expression and/or aggregation.



Modular Substitution Library : Build new genetic modules, regions or
pathways from DNA parts including regulatory elements and/or coding
sequences.

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com

Deliverables for Synthetic DNA Libraries
Pooled ss Oligo
Pooled ds
Pooled Cloning
Libraries
PCR Libraries
Libraries

Error Free
Separated
Colonies
Libraries

Up to
Up to 109
Up to 3kb
Up to 1M colonies
125nt/200nmol
transformants
Oligo mix with
PCR fragment Pool of variant
100% sequence
degenerated
Fidelity
pool with
sequences cloned verified clones in a
base(s) or triplet
variants
into vector
vector
base(s)
high or low GC
high or low GC high or low GC
regions, GC flux,
regions, GC flux, regions, GC flux,
30-80% GC, no
Complexity tandem repeats,
tandem repeats, tandem repeats,
repeats
homopolymers,
homopolymers, homopolymers,
palindromes
palindromes
palindromes
Custom


Available
Available
Vector
Amplification, Cloning and
100% sequence
Cloning and
sequencing at Sequencing at verification using
Quality
sequencing at
least 16-96
least 16-96
Next Generation
Assurance
least 16-96
clones or NGS clones or NGS to
and Sanger
clones or NGS to
to verify
verify diversity
sequencing
verify diversity
diversity
methods
Length

Pricing and Delivery Times
ErrorDiversi Oligo
Pooled
free Poole
Lengt Compl ty
or
Pooled
Deliv
Cloning Separ d ss
Clone
h
exity Analys PCR
DNA
ery
Plasmids/ ated Oligo
Library
(nt/kb Analys is by frag
Library
Time
Culture Plasm Libra
List Price
)
is Sequen ment
Priced
*
Yield
ids ries
cing Yield
Yield
0.02-0
.125





100
nmol





Inquir
e



Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com



10-1
5
Busi

ness
Days

0.1253.0

1.5-3.
0





3.0-6.
0



6.0-9.
0



>9.0













2 μg

20-100
μg/1-5ml 1-5 μg
culture



2 μg

20-100
μg/1-5ml 1-5 μg
culture





20-100
μg/1-5ml 1-5 μg
culture





20-100
μg/1-5ml 1-5 μg
culture





20-100
μg/1-5ml 1-5 μg
culture



$0.15/bp
10-1
$0.35/bp
plus
5
plus
degerated/
Busi
degerated/tr
triplet
ness
iplet price
price
Days
$0.20/bp
10-1
$0.45/bp
plus
5
plus
degerated/
Busi
degerated/tr
triplet
ness
iplet price
price
Days
20-4
$0.55/bp
0
plus

Busi
degerated/tr
ness
iplet price
Days
30-5
$0.65/bp
0
plus

Busi
degerated/tr
ness
iplet price
Days
$0.95/bp
plus
Inqui

degerated/tr re
iplet price

Related Services
 Syno®3.0 Next Generation Gene Synthesis
 Chip Based DNA Synthesis

Contact us
Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction
NJ, 08852

Tel:+1 732-230-3003
Fax:+1 609 228 5911
Web:www.synbio-tech.com
E-mail:service@synbio-tech.com


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