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Hcf .pdf


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Human Cardiac Fibroblasts
HCF from Creative Bioarray Research Laboratories are isolated from the human heart. HCF-a
are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells
in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to
fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HCF-a are guaranteed to further expand for 15 population doublings at the condition provided
by Creative Bioarray.
Quality Control
Human Primary Cardiac Fibroblasts are tested for negative expression of von Willebrand Factor
Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Cardiac
Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5
passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative
Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen
(-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Application
Human Primary Cardiac Fibroblasts can be used for the assay of cell-cell interaction, adhesion,
PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell
derivatives for desired research applications.


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