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cas9 stable cell line .pdf



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Cas9 Stable Cell Lines

CRISPR (clustered regularly interspaced short palindromic repeats) /
Cas9 (CRISPR associated protein 9) system is adapted from a naturally
occurring bacterial immune system which can protect bacteria from
damage caused by phage infection. As an unimagined tool for genome
engineering, CRISPR/Cas9 is now commonly used. Compared with ZFNs
and TALENs, CRISPR/Cas9 employs sgRNA (single guide RNA) to specify
editing which makes the system more precise, time/cost-saving and
quite valuable for high-throughput genome editing. So far, a large
number of published papers have involved the use of CRISPR/Cas9
technology.


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