Lipid Specific QD Labeling of Enveloped Viruses Using DSPE PEG Biotin .pdf
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Lipid-Specific QD Labeling of Enveloped Viruses
Quantum dots (QD) have optical properties of super bright fluorescence,
excellent photostability, narrow emission spectra and optional colors.
QD-labeled single molecules / viruses can be continuously and quickly imaged
for a long time, and more detailed information provided than other
fluorophore-labeled. QDs have rarely been used to study viral infections,
mainly due to the lack of a recognized labeling strategy. This study developed
a lipid-specific method to readily, mildly, specifically, and efficiently label
enveloped viruses with quantum dots by recognizing viral envelope lipids with
lipid-biotin conjugates (DSPE-PEG-Biotin) and recognizing these lipid-biotin
conjugates with streptavidin-quantum dot conjugates.
Such a strategy can thoroughly evade ultracentrifugation, dialysis, and
ultrafiltration processes that are indispensable for removing the cell-derived
reactive molecules, redundant functional reagents, unlabeled viruses, or
unbound QDs in many other labeling strategies. This strategy furthest
minimized and simplified the handling of viruses, making the QD labeling
milder and more convenient.
conjugates incorporating various functionalized PEG terminal, like Biotin,
Amine, Carboxylic acid, Azide, Aldehyde, Thiol, and Hydroxy.