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By combining the results of our wounding screen with miRNA expression proﬁling, we found that miR-203a was highly expressed in LBC cells and can suppress BLBC motility by silencing the transcription factor DNp63a.
Hsp68, which belongs to the Hsp70 family, was highly expressed during recovery.
Surprisingly, out of ⬃15,000 expressed sequence tags (ESTs) measured, no transcripts were upregulated during RCH, and likewise RCH had a minimal effect on the transcript signature during recovery from cold shock.
The ratio of the target gene was expressed in treated samples versus matched controls (calibrators) and normalized using the housekeeping reference gene, RpS20 (see Colinet et al., 2010 for more details).
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Expression levels of Fst are normalized against the housekeeping reference RpS20 and values are expressed as fold change relative to control (mean6SE;