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Supplementary Methods Protein expression and purification Fourier transform infrared spectroscopy Statistical analysis of fibril shape fluctuations AM-FM AFM Tables S1–S4 Figures S1–S10 Notes S1–S5 Supplementary References S1 Lamour et al.
These GAG chains are associated with nearby GAG chains through electrostatic interactions and build bridges between proteoglycan core proteins present on the collagen fibril surface (15).
Moreover, these structural changes are associated with the disruption of native contacts that when tethered prevent fibril formation.
The structure of the LCR hydrogel had characteristics of cross-β-amyloids, but, because of the reversibility and dynamism, was not nearly as stable to SDS denaturation as the yeast prion-like fibril tested.