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Published by The Company of Biologists Ltd doi:10.1242/jeb.051003 Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster Hervé Colinet1,2,*, Siu Fai Lee2 and Ary Hoffmann2 1 Earth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium and Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio21 Institute, The University of Melbourne, Parkville, Victoria 3010, Australia 2 *Author for correspondence (email@example.com) Accepted 20 September 2010 SUMMARY To protect cells from the damaging effects of environmental stresses, all organisms possess a universal stress response involving upregulation of heat shock proteins (Hsps).
We also assessed whether expression (basal and induced) of eight stress genes (hsp22, hsp23, hsp40, hsp68, hsp70Aa, hsp83, Starvin and Frost) varied post-eclosion in correspondence with changes found in cold tolerance.
On the basis of their response, we consider that Hsp60, Hsp67Ba and Hsc70-1 are not cold-inducible, whereas Hsp22, Hsp23, Hsp26, Hsp27, Hsp40, Hsp68, Hsp70Aa and Hsp83 are induced by cold.
Acclimation also inﬂuenced expression of several genes (Hsp23, Hsp70, Hsp40, Hsp68, Starvin and Frost) during recovery from cold stress but effects depended on the nature of the acclimation treatment.