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vortex mixer (Thermolyne), waterbath (Thermolyne), microplate heater (Digene), microplate shaker (Digene), luminometer (Digene), biological safety cabinet (Heraeus), dan freezer (Sansio).
The Sword ELISA Booster uses standard fluorescent microplate readers and immunoassay chemistry.
An absorbance wavelength at 580 nm was measured by using a microplate reader (Awareness Technology Inc., USA).
The optical density was determined using a POLARstar Omega microplate reader (BMG LABTECH, Offenburg, Germany).
Colorimetric results were read in a MRX Dynatech Microplate reader (Dynatech, Chantilly, VA) at a wavelength of 450 nm in 96-well high-binding Stripwell Costar EIA microplates (Costar 2592).
After dilutions when necessary, colorimetric measurements were carried out with a microplate reader (VersaMax Molecular Devices, San José, CA, USA) at 340 nm.
The homogenates were then subjected to low-spin centrifugation (500 g, 5 min, 4 °C) to allowed gentle sedimentation cells debris and 5 μL of clear supernatant were transferred (in duplicate) into a 96-well microplate, together with 250 μL of Bradford micro-assay reagent (B6916;
Protein concentration was spectrophotometrically measured at 540 nm (VersaMax™ Microplate Reader, Molecular Devise) using the Biuret method with bovine serum albumin as a standard.
After 30 min incubation, LDH activity was measured by OD450 on an iMark Microplate Absorbance Reader (Bio-Rad) and normalized to total protein concentration in the samples.
Assays were followed continuously at ambient temperature (23°C) at 412 nm for 10 min in a SpectraMax PLUS Microplate Reader (Molecular Devices Corp, Sunnyvale, CA).