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vortex mixer (Thermolyne), waterbath (Thermolyne), microplate heater (Digene), microplate shaker (Digene), luminometer (Digene), biological safety cabinet (Heraeus), dan freezer (Sansio).
The Sword ELISA Booster uses standard fluorescent microplate readers and immunoassay chemistry.
An absorbance wavelength at 580 nm was measured by using a microplate reader (Awareness Technology Inc., USA).
The optical density was determined using a POLARstar Omega microplate reader (BMG LABTECH, Offenburg, Germany).
After 30 min incubation, LDH activity was measured by OD450 on an iMark Microplate Absorbance Reader (Bio-Rad) and normalized to total protein concentration in the samples.