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Therefore, selective media (MacConkey agar) is used to select for Gram negatives, and blood agar is used to determine the total bacterial load.
Minerva Med, 92(1):29-33 [From the abstract:] "50% of the PCR positive results, obtained with serum and cerebrospinal fluid samples corresponded to patients who were true positives at clinical examination but negatives at serologic tests.
Develop your negatives to give the contrast that you prefer to print with—the exact contrast is largely a matter of personal taste.